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reporter plasmids for supertopflash  (Addgene inc)
96
Addgene inc reporter plasmids for supertopflash
(A): HEK293T cells were transiently transfected with firefly reporter plasmids for <t>SuperTopFlash</t> together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.
Reporter Plasmids For Supertopflash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supertopflash+reporter+plasmid/bio_rxiv__2025__08__02__668297-251-22-30?v=Addgene+inc
Average 96 stars, based on 1 article reviews
reporter plasmids for supertopflash - by Bioz Stars, 2026-07
96/100 stars
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8x supertopflash reporter plasmid  (Addgene inc)
96
Addgene inc 8x supertopflash reporter plasmid
(A): HEK293T cells were transiently transfected with firefly reporter plasmids for <t>SuperTopFlash</t> together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.
8x Supertopflash Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supertopflash+reporter+plasmid/pmc12103576-408-13-17?v=Addgene+inc
Average 96 stars, based on 1 article reviews
8x supertopflash reporter plasmid - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
plasmid for the 8x supertopflash reporter  (Addgene inc)
90
Addgene inc plasmid for the 8x supertopflash reporter
(A): HEK293T cells were transiently transfected with firefly reporter plasmids for <t>SuperTopFlash</t> together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.
Plasmid For The 8x Supertopflash Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supertopflash+reporter+plasmid/pm39213612-31-8-17?v=Addgene+inc
Average 90 stars, based on 1 article reviews
plasmid for the 8x supertopflash reporter - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
8x supertopflash reporter  (Addgene inc)
96
Addgene inc 8x supertopflash reporter
(A): HEK293T cells were transiently transfected with firefly reporter plasmids for <t>SuperTopFlash</t> together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.
8x Supertopflash Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supertopflash+reporter+plasmid/pm39213612-31-11-17?v=Addgene+inc
Average 96 stars, based on 1 article reviews
8x supertopflash reporter - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
supertopflash reporter  (Addgene inc)
96
Addgene inc supertopflash reporter
(A): HEK293T cells were transiently transfected with firefly reporter plasmids for <t>SuperTopFlash</t> together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.
Supertopflash Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supertopflash+reporter+plasmid/pm31550723-96-28-34?v=Addgene+inc
Average 96 stars, based on 1 article reviews
supertopflash reporter - by Bioz Stars, 2026-07
96/100 stars
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(A): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.

Journal: bioRxiv

Article Title: Pathogenic DVL frameshifting variants in Robinow syndrome disrupt WNT signaling and cellular dynamics

doi: 10.1101/2025.08.02.668297

Figure Lengend Snippet: (A): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.

Article Snippet: Cells were transfected using Lipofectamine 3000 (Invitrogen, Cat. No. L3000-008) with plasmids containing the wild type or variant DVL genes plus firefly reporter plasmids for SuperTopFlash (M50 Super 8x TOPFlash, Addgene plasmid #12456) and FOPFlash (M51 Super 8x FOPFlash, Addgene plasmid #12457) plasmid used as a negative control for “leaky” firefly luciferase transcription.

Techniques: Transfection, Construct